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本帖最后由 刘欣 于 2015-12-14 11:03 编辑
以下内容摘自犬猫细胞学,该书封面见下面照片。不知为什么很多人放着简单的细胞学不用,乐此不疲地使用DTM培养基,也不知培养了多少杂菌,还要津津乐道。谁受累帮大家翻译一下?
Dermatophytosis
This is a common infectious and often contagious disease to humans that frequently involves the superficial layers of the skin, hairs, and nails. Microsporum and Trichophyton sp. are the most common genera of dermatophytes associated with dogs and cats. The lesions typically present with focal alopecia, broken hair shafts, crusts, scales, and erythema on the head, feet, and tail of
dogs and cats (Caruso et al., 2002). Less commonly seen are raised or dermal nodules called kerions (Logan et al.,2006). A kerion forms when the infected hair follicle ruptures and both the fungus and keratin spill into the dermis, eliciting an intense infl ammatory response. Cytologic specimens reveal a pyogranulomatous inflammation with degenerate neutrophils and large epithelioid
macrophages. Arthrospores that measure 2 to 4 μm possess a thin, clear capsule (Fig. 3-20A). The arthrospores and nonstaining hyphae are associated with hair shafts, which are best visualized using clearing agents with plucked hairs (Fig. 3-20B&C) or methenamine silver staining (Fig. 3-20D) or PAS (Fig. 3-20E). Fungal culture is necessary for identifi cation. An uncommon presentation is a dermatophytic pseudomycetoma, usually seen in Persian cats, that is most often caused by Microsporum canis (Zimmerman et al.,2003). It presents as a nodular granuloma with fistulous tracts deep into subcutaneous tissues. Cytologically, this involves macrophages with abundant foamy cytoplasm and numerous multinucleated giant cells (Fig. 3-20F). Arthrospores may be present along with fungal hyphae that have an irregular shape and size and may stain variably with Romanowsky-type stains (Fig. 3-20G). Positive staining occurs with PAS and methenamine silver (Fig. 3-20H). Treatment of the nodules involves surgical excision and antifungal drugs.
FIGURE 3-20. A-E, Dermatophytosis. A, Tissue imprint. Dog. Squamous epithelium, remnant hair shaft, and mostly neutrophilic infl ammation are present along with moderate numbers of arthrospores. These basophilic, oval to elongate structures with a thin, clear capsule measure 2-3 μm in width and 2-5 μm in length. (Modifi ed Wright; HP oil.) B, Hair pluck. Low-magnifi cation view of keratincleared hair shaft with attached arthrospores. (Unstained; HP oil.) C, Hair pluck. High-magnifi cation view of keratin-cleared hair shaft demonstrating arthrospores outside and fungal hyphae within the hair. (Unstained; HP oil.) D, Tissue section. Dog. Note the black stained hyphae within the hair shaft from this tissue section of skin. Diagnosis confi rmed as M. canis by culture. (Gomori’s methenamine silver; HP oil.) E, Tissue aspirate. Dog. Dermal nodule (kerion) with multiple pink oval arthroconidia within neutrophils and extracellular. Culture results indicated Microsporum canis infection. (Periodic acid-Schiff; HP oil.) Same case F-H, Dermatophytic pseudomycetoma. Tissue aspirate. Cat. F, Several multinucleated giant cells are present in this 3-cm superfi cial mass on the lateral abdomen of a Persian cat. (Wright-Giemsa; HP oil.) 本帖最后由刘欣上半年投产 2015年-12-14 11:03 编辑以下内容摘自犬猫细胞学,该书封面见下面照片。不知为什么很多人放着简单的细胞学不用、 乐此不疲地使用DTM培养基、 也不知培养了多少杂菌、 还要津津乐道。谁受累帮大家翻译一下?
皮肤癣菌病
这是一个常见传染病和经常传染疾病给人类,频繁地涉及到皮肤,头发,表面层和指甲。小孢子菌和红色毛癣菌某地是最常见的关联与狗和猫的皮肤癣菌属。病变通常表现为局灶性秃发,碎头发轴、 壳、 鳞片,头、 脚和尾巴的红斑
狗和猫 (卡鲁索等人,2002年)。较少看到凸起或皮肤结节称为 kerions (Logan et al,2006年)。一滴血,灰心丧气形式时感染的毛囊破裂以及真菌和角蛋白渗入真皮层,从而引起激烈 infl 炎症性反应。细胞学标本揭示 pyogranulomatous 炎症与退化的中性粒细胞和大上皮样
巨噬细胞。测量 2 至 4 μ m 的 Arthrospores 具有薄、 清晰的胶囊 (图 3-20A)。Arthrospores 和 nonstaining 菌丝与头发轴,最好可视化清关代理商用拔的毛 (图 3-20B & C) 或六胺银染色 (图 3-托德) 或 PAS (图 3-20E) 相连。真菌培养是保证书阳离子的必要条件。罕见的演示文稿是真菌 pseudomycetoma,通常见于波斯猫,这通常由犬小孢子菌 (齐默尔曼 et al.,2003年) 造成的。它表现为结节性肉芽肿与瘘管型大片深入皮下组织。细胞学,这涉及到丰富的泡沫细胞质和众多多核巨细胞 (图 3-20F) 的巨噬细胞。Arthrospores 可能会出现与真菌的菌丝,有不规则的形状,大小和可染色易变,Romanowsky 型污渍 (图 3-tog).PAS 和六胺银 (图 3-toh) 出现阳性染色。结核的治疗包括手术切除和抗真菌药物。
图 3-20。A-E,皮肤癣菌病。一、 组织的印记。狗。鳞状上皮、 残头发轴,主要是中性粒细胞 infl 发炎目前和及其适度页码的 arthrospores。这些嗜碱性,椭圆形拉长薄、 清晰的胶囊结构测量 2-3 微米的宽度和长度在 2-5 μ m。(修饰埃德赖特;HP 的油)。B、 头发摘下。低 magnifi 阳离子的附加 arthrospores keratincleared 头发轴视图。(污泥而不染;HP 的油)。C、 头发摘下。角蛋白清除头发轴展示 arthrospores 高 magnifi 阳离子视图在头发内的外部和真菌菌丝。(污泥而不染;HP 的油)。D、 组织切片。狗。请注意黑色染色的菌丝在毛干从皮肤本组织部分内。M.犬由文化作为诊断保兑。(去污的六胺银;HP 的油)。E、 组织抽吸。狗。与多个粉红色的椭圆形 arthroconidia 中性粒细胞内和细胞外的皮肤结节 (癣)。培养结果表明犬小孢子菌感染。(过碘酸-希夫;HP 的油)。同一案件 F-H,真菌 pseudomycetoma。组织抽吸。猫。F,几个多核巨细胞是存在于这 3 厘米 superfi 社会大众侧腹部的一只波斯猫。(瑞氏;HP 的油)。
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